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Request for Quotation: Next-generation sequencing services on fish tissues

August 7 @ 5:00 pm EDT

Solicitation #: 140G0320Q0170

“The US Geological Survey is investigating contaminant Polychlorinated biphenyl (PCB) health effects thresholds for Pacific herring (Clupea pallasii). Herring and other fishes in Puget Sound have high tissue concentrations of PCBs, yet we know little about related biological impacts. The USGS will conduct a controlled laboratory study, first exposing herring to environmentally relevant concentrations of PCBs through their food, then introducing a disease challenge to PCB-exposed fish. Sub-lethal genetic responses in herring will be assessed following PCB exposure by using an RNA-seq experiment, and immunological impacts of PCB exposure on disease challenged-fish will be determined from functional responses using RNA-seq in lymphatic tissues. To facilitate differential expression analysis, we are requesting next-generation sequencing services on fish tissues.”

Due date: 07 August 2020, 5:00 p.m. EDT