« All Events

  • This event has passed.

Request for Quotes: Salmon Genotyping

September 20 @ 3:00 pm

Solicitation #: 12305B22R0016

“The USDA Agricultural Research Service requires single nucleotide polymorphism (SNP) genotyping, microsatellite testing, and DNA extraction from Atlantic salmon tissues. Atlantic salmon tissue samples will consist of either fin clips or muscle tissue that has been preserved in ethanol or freeze dried. Some DNA samples may require the extracted DNA to be aliquoted out into 3 lots. One lot will be used for the required genotyping, and the remaining two lots from each sample will be placed in uniquely identified, human readable, sealed 96 or 384 well plates and returned to the USDA along with a key linking the unique sample ID to plate and well locations.

Atlantic salmon DNA genotyping will be completed using the N.A. Atlantic Salmon50K Axiom myDesign TG array for single nucleotide polymorphism (SNP) genotyping in the 384-well format (Thermo-Fisher SKU number 551260 or Neogen SKU number 478) on the GeneTitan Instrument or equal. Deliverables will include the genotyping data for all the samples and the raw data files in .cel format (CEL files). Unused tissue samples and DNA samples will be sent back to the government. Contractor shall provide all services, supplies, equipment and facilities necessary to provide the deliverable specified in accordance with the terms, conditions, and specifications contained in this document.”

Due date: 20 September 2022, 3:00 p.m. EDT