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Webinar: Automated sample preparation for reproducible clinical proteomics

May 19 @ 11:00 am - 12:00 pm

“Mass spectrometry (MS)-based proteomic technologies are increasingly applied in a clinical context for disease classification and diagnostics, however their standardized use is still prevented by several bottlenecks in sample handling. Here, I will present how we integrated AFA-based ultrasonication in an automated proteomic workflow to enable routine, robust and comprehensive proteome analysis with minimal hands-on time. This can be applied to cells, fresh tissue and FFPE tissue, thus spanning the full range of clinical sample types and indicating universal utility. Moreover, 96 samples can be processed simultaneously, ensuring throughput under standardized conditions. Sensitivity of the approach will be shown from low-input experiments starting from 100-1000 cells. Data will be shown of the implementation of this pipeline to a cohort of 96 ependymoma tumor samples, where we integrated the obtained proteomic data with other omics layers allowing individualized molecular profiling and disease sub-classification.”