Combine Solicitation – DNA Genome Analysis System

General Information

Document Type: Combine Solicitation
Solicitation Number: NHLBI-PB(HL)-2007-008-1DDC
Posted Date: Dec 08, 2006
Original Response Date: Dec 15, 2006
Current Response Date: Dec 15, 2006
Original Archive Date: Dec 30, 2006
Current Archive Date: Dec 30, 2006
Classification Code: 66 — Instruments & laboratory equipment
Naics Code: 334516 — Analytical Laboratory Instrument Manufacturing

Contracting Office Address

Department of Health and Human Services, National Institutes of Health, National Heart, Lung and Blood Institute, Rockledge Dr. Bethesda, MD, Office of Acquisitions 6701 Rockledge Dr RKL2/6100 MSC 7902, Bethesda, MD, 20892-7902, UNITED STATES

Description

THIS IS A COMBINED SYNOPSIS / SOLICITATION NOTICE.       This is a combined synopsis / solicitation for commercial items, prepared in accordance with the format of FAR 12.6, as supplemented with additional information included in this notice.       This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued.      

The reference solicitation number is:       NHLBI-PB(HL)-2007-008-1/DDC.

This solicitation is issued as a Request for Quotation (RFQ).

This acquisition is being conducted under FAR Part 13, simplified acquisition procedures and the resultant purchase order will include all applicable provisions and clauses in effect through the Federal Acquisition Circular 05-13.             The total contracted dollar amount, including options will not exceed $5 million.    

The acquisition is being conducted as a full and open competitive, with no set aside restrictions.     The North American Classification System (NAICS) code applicable to this requirement is 334516 and the associated small business size standard is 500 employees.

The Government?s anticipated delivery date is not later than January 15, 2007.    

The delivery point is the National Institutes of Health, National Heart, Lung, and Blood Institute (NHLBI), Laboratory of Molecular Immunology, Bethesda, Maryland.        

Market research conducted by the Government has determined that DNA Genome Analysis System is manufactured in sufficient quantities by at least three (3) manufacturers which confirm the commercial market.

The National Institutes of Health (NIH), National Heart, Lung, and Blood Institute (NHLBI), Office of Acquisition (OA),     for the NHLBI, Laboratory of Molecular Immunology, intend to award a single, fixed priced purchase order for the purchase a DNA Genome Analysis System.      

The specifications of the DNA Genome Analysis System are as follows: (1) It does not require complicated sample preparation. It should only require one-step linker ligation and one-step PCR amplification for analysis using the system; (2) one run of sequencing analysis should yield more than five millions 25-bp reliable sequence tags in less than 48 hours on machine; (3) The sensitivity of the system should be able to detect less than 100 nanograms of Input ChIP DNA sample.      

The Laboratory of Molecular Immunology study the regulation of human gene expression by chromatin that is formed by histone-DNA interaction.       The structure of chromatin is regulated by multiple post-translational histone modifications.     In order to understand how histone modifications regulation gene expression.     The research study needs to determine the distribution of these modified histones in the genome.       For this purpose, the research will use chromatin immunoprecipitation (ChIP) to pull down DNA associated with modified histones, followed by the identification of a 25bp tag sequence from each ChIP DNA molecule.

http://www.fbo.gov/spg/HHS/NIH/NHLBI/NHLBI%2DPB%28HL%29%2D2007%2D008%2D1DDC/Combine%20Synopsis%5FSolicitation.html