ChromaWizard: An open-source image analysis software for multicolor fluorescence in situ hybridization analysis

In this brief article published in Cytometry Part A, researchers at the Austrian Centre for Industrial Biotechnology present their open-source multiplex fluorescence in situ hybridization (M‐FISH) software for chromosome painting. The tool—ChromaWizard—acts as a free and open-source option for hybridization analysis, integrating “image processing, multicolor integration, chromosome separation, and visualization with false color assignments.” The software can handle images in TIFF, PNG, and JPEG formats and provides robust visualization tools. The authors conclude that ChromaWizard “allows direct inspection of the original hybridization signals and enables either manual or automatic assignment of colors, making it a functional and versatile tool that can also be used for other multicolor applications.”

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