Enzyme immunoassay for measuring aflatoxin B1 in legal cannabis

In this 2020 paper published in the journal Toxins, Di Nardo et al. of the University of Turin present their findings from adapting an existing enzyme immunoassay to cannabis testing for purposes of more accurately detecting mycotoxins, including aflatoxins such as aflatoxin B1 (AFB1). Citing benefits such as fewer steps, more cost-efficient equipment, and fewer training level demands, the authors viewed the application of enzyme immunoassay to testing cannabis for mycotoxins a worthy endeavor. Di Nardo et al. discuss at length how they converted an immunoassay for measuring aflatoxins in eggs to one for the cannabis substrate, as well as the various challenges and caveats associated with the resulting methodology. In comparison to techniques such as ultra-high performance liquid chromatography coupled to high resolution tandem mass spectrometry, the authors concluded that enzyme immunoassay more readily allows for “wide applications in low resource settings and for the affordable monitoring of the safety of cannabis products, including those used recreationally and as a food supplement.”

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