A sustainable approach for the reliable and simultaneous determination of terpenoids and cannabinoids in hemp inflorescences by vacuum-assisted headspace solid-phase microextraction

In this 2022 journal article published in Advances in Sample Preparation, Capetti et al. of Università di Torino investigate the advantages and disadvantages that vacuum-assisted headspace solid-phase microextraction (Vac-HSSPME) may lend to the process of Cannabis inflorescence preparation and subsequent testing, by gas chromatography–mass spectrometry (GC-MS), in order to accurately characterize both terpenoid and cannabinoid profiles. After providing an introduction on the topic, the authors describe the materials and methods used, as well as the results of their study. They conclude that “compared to standard HSSPME, vacuum conditions in the headspace ensure the fast recovery of cannabinoid markers at considerably lower sampling temperature (i.e., 90°C) that do not discriminate the most volatile fraction nor cause the formation of artifacts when the sampling time is minimized.” They add that, in combination with GC-MS, Vac-HSSPME can prove to be “fast, totally automatable, and solvent-free,” representing a more green analytical approach to Cannabis inflorescence testing.

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